Properties
Destabilized, Membrane Anchored Gaussia Luciferase
Developed by Xactagen scientists, this reporter technology gives our cells distinct advantages in most in vitro and in vivo applications.
  • Anchored to the cell membrane and extracellularly displayed, the enzyme remains associated with the cell, but does not require cell lysis for reporter assays
  • It retains the exceptional flash activity of secreted Gaussia luciferase, but is further intrinsically stabilized for unsurpassed glow activity that outperforms other luciferases
  • Exceptional for in vitro applications, intrinsic stabilization for glow activity is uniquely significant for in vivo applications with whole-animal imaging where chemical “stabilizers” typical of competitive products are not practical.
  • The destabilized enzyme (reduced half life of the protein) enables analysis of stimulants and inhibitors without interference from accumulated enzyme activity.
  • High Z-factor: Background is very low, while expression levels span 7 to 8 orders of magnitude, providing sensitivity for both very weak and very strong promoters
  • Destabilized: Half life of 4-5 hrs, so effects of stimulants and inhibitors are not masked by lingering reporter activity
  • “User Friendly” assays: experimental samples may be frozen and assayed when more convenient, minimal and simple assay procedures amenable to bench top scientists/technicians or automated liquid handlers
Membrane-anchored Gaussia luciferase is available with both our standard reporter cells and endogenous reporter cells.

For more details about our reporters’ performance and flash and glow characteristics, see our Technology pages.